The throttle body mounting stud arrangement is totally different between the two. Also the 5litre tb has 2 studs whereas the ls1 has 4. You're going to need to make up an adaptor plate that suits the ls1 tb mating face on one side, and the 5litre manifold on the other. This adaptor plate would also have to be internally tapered to suit the bore size of tb down to the inlet manifold, otherwise your inlet air charge will be like hitting a wall ubstruction. Better though would be to enlarge and port manifold to suit but this would require its removal. You may also need to customise the throttle cable holder and linkage on the ls1 tb.
No you wont need to use the ls1 maf sensor. You can, but its not necessary. This is because any increase in inlet air charge flow you may gain will still be within the scale of the stock 5litre maf.